Evaluation of Renal-Hepatic Functional Indices and Blood Pressure Based on the Progress of Time in a Rat Model of Chronic Kidney Disease

BACKGROUND Chronic kidney disease (CKD) is defined as either kidney damage or a decline in renal function as determined by a decreased glomerular filtration rate (GFR) for three months or longer. CKD is an important risk factor for mortality. OBJECTIVES The aim of this study was to evaluate the effects of CKD on renal-hepatic functional indices and blood pressure in 5/6 nephrectomized (5/6 Nx) rats over the course of nine months. MATERIALS AND METHODS Male Wistar rats were subjected to either 5/6 Nx or sham operations (n = 8). Members of the sham group underwent the same procedure without surgical reduction of the kidney mass. For all animals, body weight (BW), serum creatinine (Cr), blood urea nitrogen (BUN), alanine transaminase (ALT), and aspartate transaminase (AST) levels were measured before and after surgery. After two-, three-, six-, and nine-month intervals, blood was collected to assay renal and hepatic functional indices. Tail-cuff blood pressure was recorded in each month after surgery. RESULTS BW was lower for the 5/6 Nx group rats after the operations compared with the BW of those in the sham operation group. Furthermore, the 5/6 Nx group showed elevations in blood pressure, Cr, BUN, ALT, and AST levels compared with the sham group over the course of time. CONCLUSIONS In summary, CKD induced by the 5/6 Nx model caused hypertension and increased serum levels of Cr, BUN, ALT, and AST. These changes are augmented by the progress of time.